![]() Vacuum Adapters (Cat.# A1331) are required for use of the vacuum format with the Wizard® SV Gel and PCR Clean-Up System and must be purchased separately. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation, Purified DNA routinely achieves 700 bases with >98% accuracy in automated fluorescent sequencing. Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified RNA. This membrane-based system can bind up to 40μg of DNA, and allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed. Up to 95% recovery is achieved depending upon the DNA fragment size and purified DNA can be eluted in as little as 15μl. PCR products are commonly purified to remove excess nucleotides and primers. The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Location: Training & Education website, Tools & Aids tab. The AU480 In-Lab Training Manual provides instructions for training additional operators in your facility, a competency exercise and a competency exercise key. Choose from clear or red dyed formulations with and without magnesium chloride (MgCl 2) or a pre-prepared readymix or master mix with buffer and dNTPs.Wizard® SV Gel and PCR Clean-Up System Details most frequently asked questions: Startup, Racks and Sample Containers, Maintenance, Troubleshooting and as needed tasks. Use the table below to select an appropriate mix of Taq DNA polymerase for your reaction conditions. The enzyme is supplied at 5 units/µL and comes with an optimized 10x reaction buffer. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing. It has 5'→3' DNA polymerase activity and 5'→3' exonuclease activity. The enzyme has a molecular weight of approximately 94 kDa by SDS-PAGE with no detectable endonuclease or exonuclease activity. It is able to withstand repeated heating to 95 ☌ (as is demanded by the PCR technique) without significant loss of activity. The enzyme is in a recombinant form, expressed in E. It is commonly used to amplify DNA fragments in PCR. Taq DNA polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus.
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